and for DCT project from Pine 큰 Whirl Sometimes.

Hello, everyone.

I'm honey Fattah,

and I'm the group leader of femme screaming molecular Philo

Scott, who, which is located at continuam Institute for the

Science of light, and, uh, it is funded by McSplunk Society.

We are relatively new group.

which was officially started in January, 2020.

So how I ended up in the beautiful Franconia, I did my PhD at NPQ in Munich, and after graduation

I spent a short period of time at University of Constance, Oxford, and Harvard, and then

I returned to NPQ and became a group letter there.

So my research in Munich was focused on sub-circuit pulse generation,

attachment and femtosecond spectroscopy, and I also did a lot of laser development.

So since October, 2019 I moved to Tranquilia Max Planck Institution

for Science of Light in Erlangen and established an independent research group here.

So here I am focused to employ the technologies that were developed in my labOOKP.

We have several other projects in the lab, but today and in this talk,

I will just focus on the microscopy project.

Light is one of the most important tools for human beings to understand its surroundings from

it's surrounding, from giant galaxies down to small molecules.

Abbey, in 19th century, discovered that

the spatial resolution in any optical imaging tool,

such as microscopes or telescopes,

is defined by the diffraction limit of light which is

proportional to the wavelength of light.

This means that if we would like to observe smaller objects,

we need to use light at shorter wavelengths.

Based on this, he defined a 200 nanometer as the limit for the special resolution of

optical imaging tools.

This is half of the highest frequency which is visible to bare eyes.

The barrier has been overcome by super resolution microscopy.

In a conventional microscope, the molecules in the sample are labeled with fluorescent

dye molecules which are excited by a laser beam. By detecting the fluorescence light

and raster scanning of the light laser over the sample, one can make an image of the sample.

In super resolution microscopy, which is pioneered by Stefan Hele and Eric Be laying, the special

structure apply transition of the dye molecules or the time dimension is used to make sub

diffraction limit images down to 80 nanometer spatial resolution. This is a very beautiful

work, which was praised by a Nobel Prize in 2014 and it is opening new frontiers in biology.

However, also in super resolution microscopy, the molecules have to be labeled, and most

of the time the labels are much bigger than the molecules that you would like to observe

And therefore they preserve the original function

of the molecules.

It is also very cumbersome to gain chemical information

in this kind of microscopy.

So then there is this question that, if there is any way

to obtain the image without labeling the molecules?

Of course, one can try to look at the light which

is emitted or absorbed by the molecules themselves.

We know that molecules absorb and emit light

at the characteristic frequencies.